The total volume of the BioChamber 2000 (BC-2000) is 2060 mL.

The total volume of the BioChamber 250 (BC-250) is 325 mL.

The volume displaced by the shaft of the O₂ Gas Sensor when placed into the BioChamber is 15 mL.

The volume displaced by the shaft of the CO₂ Gas Sensor when placed into the BioChamber is 35 mL.

To get the total volume of one of the BioChambers, subtract the sensor shaft volume from the total volume of the chamber to get the actual volume during data collection.