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Experiments​

Cell Respiration (CO2 and O2)

Experiment #11D from Biology with Vernier

Education Level
High School
College

Introduction

Cell respiration refers to the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely if sufficient oxygen is available according to the following equation:

{{\text{C}}_{\text{6}}}{{\text{H}}_{{\text{12}}}}{{\text{O}}_{\text{6}}} + {\text{ 6 }}{{\text{O}}_{\text{2}}}\left( {\text{g}} \right) \to {\text{6 }}{{\text{H}}_{\text{2}}}{\text{O }} + {\text{ 6 C}}{{\text{O}}_{\text{2}}}\left( {\text{g}} \right) + {\text{ energy}}

All organisms, including plants and animals, oxidize glucose for energy. Often, this energy is used to convert ADP and phosphate into ATP. Peas undergo cell respiration during germination. Do peas undergo cell respiration before germination? Using your collected data, you will be able to answer the question regarding respiration and non-germinating peas.

Using the CO2 Gas Sensor and O2 Gas Sensor, you will monitor the carbon dioxide produced and the oxygen consumed by peas during cell respiration. Both germinating and non-germinating peas will be tested. Additionally, cell respiration of germinating peas at two different temperatures will be investigated.

Objectives

In this experiment, you will

  • Measure gas production.
  • Study the effect of temperature on cell respiration.
  • Determine whether germinating peas and non-germinating peas respire.
  • Compare the rates of cell respiration in germinating and non-germinating peas.

Sensors and Equipment

This experiment features the following sensors and equipment. Additional equipment may be required.

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This experiment is #11D of Biology with Vernier. The experiment in the book includes student instructions as well as instructor information for set up, helpful hints, and sample graphs and data.

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