Sugar Fermentation in Yeast
Experiment #6B from Agricultural Science with Vernier
- Subject
- Agricultural Science
Introduction
Yeast are able to metabolize some foods, but not others. In order for an organism to make use of a potential source of food, it must be capable of transporting the food into its cells. It must also have the proper enzymes capable of breaking the food’s chemical bonds in a useful way. Sugars are vital to all living organisms. Yeast are capable of using some, but not all sugars as a food source. Yeast can metabolize sugar in two ways, aerobically, with the aid of oxygen, or anaerobically, without oxygen.
In this lab, you will try to determine whether yeast are capable of metabolizing a variety of sugars. Although the aerobic fermentation of sugars is much more efficient, in this experiment we will have yeast ferment the sugars anaerobically. When the yeast respire aerobically, oxygen gas is consumed at the same rate that CO2 is produced—there would be no change in the gas pressure in the test tube. When yeast ferment the sugars anaerobically, however, CO2 production will cause a change in the pressure of a closed test tube, since no oxygen is being consumed. We can use this pressure change to monitor the fermentation rate and metabolic activity of the organism.
Objectives
- Use a gas pressure sensor to measure the pressure change caused by carbon dioxide released during fermentation.
- Determine the rate of fermentation.
- Determine which sugars yeast can metabolize.
Sensors and Equipment
This experiment features the following sensors and equipment. Additional equipment may be required.
Option 1
Option 2
Ready to Experiment?
Ask an Expert
Get answers to your questions about how to teach this experiment with our support team.
- Call toll-free: 888-837-6437
- Chat with Us
- Email support@vernier.com
Purchase the Lab Book
This experiment is #6B of Agricultural Science with Vernier. The experiment in the book includes student instructions as well as instructor information for set up, helpful hints, and sample graphs and data.
